2
1st PCR was executed by utilizing exon 4a-specific ahead primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was done by making use of the main PCR product to be a template with all the nested Ex4a(+)WT1 cDNA-specific ahead primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') along with the nested adaptor inner primer (5'-CGCGGATCCGAATTAA