2
(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was firm by RT CR working with Ex4-F and Ex6-R primer pair that amplifies both Ex4a(+)WT1 and main WT1 isoforms in two diverse WT1-expressing cancer cells (LU99B and K562). (B-D) Actin is made use of as an inner handle. Success are agent of 3 unbiased experiments.(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was determined by RT CR utilizing